Effect Of Interferon-γ and TNF-α on MUCl MUCIN Expression in Ovarian Carcinoma Cell LinesReportar como inadecuado




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Disease Markers - Volume 12 1994, Issue 1, Pages 43-50

Department of Obstetrics And Gynaecology, University of Queensland, Clinical Sciences Building, Royal Brisbane Hospital, Henton 4029, Australia

Received 26 March 1994

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In view of the potential uses of cell surface tumour associated antigens in novel anticancer treatment. a study was designed to investigate whether the biological response modifiers interferon-gamma IFN-γ and tumour necrosis factor-alpha TNF-α could effect the expression of an epitope on the tumour associated MUC I epithelial mucin. Four ovarian carcinoma cell lines showing high OAW42 and GG and low JAM and PEO1 basal expression of MUC1 were treated with 10-1000 U-mL of IFN-γor TNF-α for one or five days. Changes in MUC1 expression in cells exposed to IFN-γ or TNF-α were monitored using an ELISA technique with the monoclonal antibody BC2 which reacts with a core protein epitope on the MUC1 mucin, and then corrected for the number of viable cells present. TNF-α had little effect on MUC1 expression, but one or five days exposure to IFN-γ significantly increased MUC1 expression p < 0.01 in all cell lines including the two cell lines that initially showed little or no expression.





Autor: Sean Clark, Michael A. Mcguckin, Terry Hurst, and Bruce G. Ward

Fuente: https://www.hindawi.com/



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