Genetic Polymorphism of β-Lactoglobulin in Native Sheep from the Island of PagReportar como inadecuado

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Food Technology and Biotechnology, Vol.40 No.1 March 2002. -

Milk samples from 248 Pag ewes, belonging to 14 different flocks and located through the Pag Island Croatia, were analyzed by isoelectrofocusing and PCR-RFLP. Two genetic variants A and B and three genotypes AA, AB and BB of β-lactoglobulin have been identified. According to the allele frequency A=0.48, B=0.52 and occurrence of genetic variants, the Pag breed is similar to other Mediterranean dairy sheep breeds. The observed genotype frequencies at the β-lactoglobulin locus AA=0.185, AB=0.589 and BB=0.226 were significantly different from those expected from Hardy-Weinberg equilibrium. In comparison with 12 different studies related to Mediterranean dairy sheep populations, the significant departure from Hardy-Weinberg was also obtained in Valle del Belice sheep. However, the sample size required for detection of the same amount of departure from the Hardy-Weinberg as it was observed in Pag sheep was only sufficient in four studies including Pag sheep. The superiority of the AB β-lactoglobulin genotype for milk production could be one of possible reasons for the observed excess of heterozygotes and allele frequencies at intermediate level.

β-lactoglobulin polymorphism; Pag sheep; Pag cheese

Autor: Vlatka Cubric-Curik - ; Dairy Science Department, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, HR-10 000 Zagr



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