Altered Expression Levels of MMP1, MMP9, MMP12, TIMP1, and IL-1 as a Risk Factor for the Elevated IOP and Optic Nerve Head Damage in the Primary Open-Angle Glaucoma PatientsReportar como inadecuado




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BioMed Research International - Volume 2015 2015, Article ID 812503, 8 pages -

Research Article

Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-647 Lodz, Poland

Department of Cancer Biology, AFCRI, Perelman School of Medicine, University of Pennsylvania, PA 19104, USA

Department of Biochemistry and Molecular Biology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA

Department of Ophthalmology, Medical University of Warsaw, 03-709 Warsaw, Poland

Received 16 December 2014; Revised 22 April 2015; Accepted 23 April 2015

Academic Editor: Alvin L. Young

Copyright © 2015 Lukasz Markiewicz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of presented work was to analyze the impact of particular polymorphic changes in the promoter regions of the -1607 1G-2G MMP1 -1562 C-T MMP9 -82 A-G MMP12 -511 C-T IL-1β, and 372 T-C TIMP1 genes on their expression level in POAG patients. Blood and aqueous humor samples acquired from 50 patients with POAG and 50 control subjects were used for QPCR and protein levels analysis by ELISA. In vivo promoter activity assays were carried on HTM cells using dual luciferase assay. All studied subjects underwent ophthalmic examination, including BCVA, intraocular pressure, slit-lamp examination, gonioscopy, HRT, and OCT scans. Patients with POAG are characterized by an increased mRNA expression of MMP1, MMP9, MMP12, and IL-1β genes as compared to the control group . Aqueous humor acquired from patients with POAG displayed increased protein expression of MMP1, MMP9, MMP12, and IL-1β compared to the control group . Allele -1607 1G of MMP1 gene possesses only 42,91% of the -1607 2G allele transcriptional activity and allele -1562 C of MMP9 gene possesses only 21,86% of the -1562 T allele. Increased expression levels of metalloproteinases can be considered as a risk factor for the development of POAG.





Autor: Lukasz Markiewicz, Dariusz Pytel, Bartosz Mucha, Katarzyna Szymanek, Jerzy Szaflik, Jacek P. Szaflik, and Ireneusz Majsterek

Fuente: https://www.hindawi.com/



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