The Stimulatory Effect of Cerebral Intraventricular Injection of cNPY on Precocial Feeding Behavior in Neonatal Chicks Gallus domesticusReportar como inadecuado




The Stimulatory Effect of Cerebral Intraventricular Injection of cNPY on Precocial Feeding Behavior in Neonatal Chicks Gallus domesticus - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Neuropeptide Y NPY is one of the most potent stimulants of food intake in many animals. Most of the supporting evidence for the effects of NPY has been gathered in mammalian species using porcine NPY. To investigate the effects of NPY on precocial feeding initiation in chicks, we firstly used chicken NPY cNPY to study its role in food intake and spontaneous activities in 3-day-old male chicks. Food intake was monitored at different times after intracerebroventricular ICV injection of cNPY 2.5, 5.0 or 10.0 μg-10 μL and anti-cNPY antibody anti-cNPY 1:9000, 1:3000 or 1:1000 in dilution. cNPY given at different doses significantly increased food intake at 30 min, 60 min, 90 min and 120 min after injection. Chicks treated with 5.0 μg-10 μL of cNPY showed a maximal 4.48 fold increase in food intake comparing to the control at 30 min. There is still more than 2 fold increase in food intake at 120 min after injection of cNPY. Food intake was significantly inhibited by a single ICV injection of anti-cNPY diluted to 1:9000 60% inhibition, 1:3000 92% inhibition, and 1:1000 95% inhibition at 30 min with 1:1000 being the maximally effective concentration. The inhibitory effects of anti-cNPY diluted to1:9000, 1:3000, 1:1000 at 120 min post ICV injection were 22%, 42% and 46%, respectively. But ICV of anti-cNPY 1:3000 in dilution did not block the orexigenic effect of 2.5 μg-10 μL of cNPY. ICV injection of different concentrations of cNPY increases locomotor activity in a dose-dependent manner while ICV anti-cNPY greatly decreased the distance moved by each chick compared to control groups. Taken together, our results demonstrated that cNPY has a promoting effect on chick food intake and locomotor activity, and that endogenous cNPY might play a positive role in regulating precocial feeding behavior in newly hatched chicks.



Autor: Guiqian Chen, Feifei Yang, Taofen Wu, Junfang Jiang, Weidong Zhou

Fuente: http://plos.srce.hr/



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