The Use of DNA Barcoding on Recently Diverged Species in the Genus Gentiana Gentianaceae in ChinaReportar como inadecuado

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DNA barcoding of plants poses particular challenges, especially in differentiating, recently diverged taxa. The genus Gentiana Gentianaceae is a species-rich plant group which rapidly radiated in the Himalaya-Hengduan Mountains in China. In this study, we tested the core plant barcode rbcL + matK and three promising complementary barcodes trnH-psbA, ITS and ITS2 in 30 Gentiana species across 6 sections using three methods the genetic distance-based method, Best Close Match and tree-based method. rbcL had the highest PCR efficiency and sequencing success 100%, while the lowest sequence recoverability was from ITS 68.35%. The presence of indels and inversions in trnH-psbA in Gentiana led to difficulties in sequence alignment. When using a single region for analysis, ITS exhibited the highest discriminatory power 60%-74.42%. Of the combinations, matK + ITS provided the highest discrimination success 71.43%-88.24% and is recommended as the DNA barcode for the genus Gentiana. DNA barcoding proved effective in assigning most species to sections, though it performed poorly in some closely related species in sect. Cruciata because of hybridization events. Our analysis suggests that the status of G. pseudosquarrosa needs to be studied further. The utility of DNA barcoding was also verified in authenticating ‘Qin-Jiao’ Gentiana medicinal plants G. macrophylla, G. crassicaulis, G. straminea, and G. dahurica, which can help ensure safe and correct usage of these well-known Chinese traditional medicinal herbs.

Autor: Juan Liu, Hai-Fei Yan, Xue-Jun Ge



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