Effects of Nogo-A Silencing on TNF-α and IL-6 Secretion and TH Downregulation in Lipopolysaccharide-Stimulated PC12 CellsReportar como inadecuado




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BioMed Research International - Volume 2015 2015, Article ID 817914, 6 pages -

Research Article

Neurology Department, People’s Hospital of Zengcheng City Boji Hospital Affiliated to Sun Yat-sen University, Guangzhou 511300, China

Neurology Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China

Received 8 May 2015; Accepted 25 June 2015

Academic Editor: Sun-On Chan

Copyright © 2015 Jianbin Zhong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Parkinson’s disease PD is a common degenerative disease that lacks efficient treatment. Myelin-associated neurite outgrowth inhibitor A Nogo-A is relevant with inhibition of nerve regeneration and may play vital role in pathogenesis of PD. The study aimed to establish the shRNA expression plasmids of Nogo-A gene and explore the regulatory effects of Nogo-A silencing on the expression of inflammation factor tumor necrosis factor-alpha TNF-alpha and interleukin-6 IL-6 as well as tyrosine hydroxylase TH in lipopolysaccharide- LPS- stimulated rat PC12 cells. The results showed that both mRNA and protein levels of Nogo-A in pGenesil-nogoA-shRNA group were downregulated. The viabilities of PC12 cells decreased with increase of LPS concentrations. LPS significantly increased the supernatant TNF-alpha and IL-6 concentrations and reduced TH protein expression in PC12 cells, while silencing Nogo-A could block these effects. These results suggested that LPS can activate PC12 cells to secrete inflammatory cytokines and lower the TH expression, which can be regulated by Nogo-A gene silencing. Nogo-A silencing might provide new ideas for PD treatment in the future.





Autor: Jianbin Zhong, Shengnuo Fan, Zhenwen Yan, Songhua Xiao, Limei Wan, Chibang Chen, Simin Zhong, Lu Liu, and Jun Liu

Fuente: https://www.hindawi.com/



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