Menoprogen, a TCM Herbal Formula for Menopause, Increases Endogenous E2 in an Aged Rat Model of Menopause by Reducing Ovarian Granulosa Cell ApoptosisReportar como inadecuado

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BioMed Research International - Volume 2016 2016, Article ID 2574637, 12 pages -

Research Article

Traditional Chinese Medicine Department, Nanjing University of Chinese Medicine, Nanjing 210046, China

Institute of Botany, Chinese Academy of Science, Nanjing, Jiangsu 210014, China

Hainan Medical College, Haikou, Hainan 571101, China

Department of Pharmacy Practice, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, USA

Beijing Clinical Services Center, No. 103 Chaoyang North Road, Beijing 100123, China

Received 9 November 2015; Revised 12 January 2016; Accepted 17 January 2016

Academic Editor: Rosaria Scudiero

Copyright © 2016 Yu Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The effect of Menoprogen MPG on ovarian granulosa cell GC apoptosis was investigated in vitro and in vivo in an aged rat model of menopause. Intragastric administration of Menoprogen or estradiol valerate to 14-month-old senile female rats for eight weeks increased plasma levels, as well as the weight of both ovarian and uterine tissues. Flow cytometric FCM analysis of isolated GCs from MPG-treated aged rats showed reductions in the ratio and apoptotic peaks. Isolated GCs also exhibited an increase in cell size and the number of cytoplastic organelles and intracellular gap junctions, the reappearance of secretory granules, and a lack of apoptotic bodies as determined by TEM. Results from a TdT-mediated dUTP nick end-labeling TUNEL assay revealed a reduction in TUNEL-positive GCs after MPG treatment. Immunohistochemical analysis showed a downregulation of proapoptotic Bax proteins and an upregulation of antiapoptotic Bcl-2 proteins. The addition of MPG-medicated serum to the media of cultured GCs also reduced cadmium chloride-induced apoptosis and downregulated caspase-3 protein expression. This work demonstrates that Menoprogen inhibits GC apoptosis in aged female rats and thereby increases production. This represents a novel mechanism of action for this herbal medicine in the treatment of menopausal symptoms.

Autor: Yu Li, Hong Ma, Ye Lu, B. J. Tan, L. Xu, Temitope O. Lawal, Gail B. Mahady, and Daniel Liu



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