Newcastle Disease Virus V Protein Targets Phosphorylated STAT1 to Block IFN-I SignalingReportar como inadecuado




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Newcastle disease virus NDV V protein is considered as an effector for IFN antagonism, however, the mechanism remains unknown. In this study, the expression of STAT1 and phospho-STAT1 in cells infected with NDV or transfected with V protein-expressing plasmids were analyzed. Our results showed that NDV V protein targets phospho-STAT1 reduction in the cells depends on the stimulation of IFN-α. In addition, a V-deficient genotype VII recombinant NDV strain rZJ1-VS was constructed using reverse genetic technique to confirm the results. The rZJ1-VS lost the ability to reduce phospho-STAT1 and induced higher expression of IFN-responsive genes in infected cells. Furthermore, treatment with an ubiquitin E1 inhibitor PYR-41 demonstrated that phospho-STAT1 reduction was caused by degradation, but not de-phosphorylation. We conclude that NDV V protein targets phospho-STAT1 degradation to block IFN-α signaling, which adds novel knowledge to the strategies used by paramyxoviruses to evade IFN.



Autor: Xusheng Qiu, Qiang Fu, Chunchun Meng, Shengqing Yu, Yuan Zhan, Luna Dong, Cuiping Song, Yingjie Sun, Lei Tan, Shunlin Hu, Xiaoqua

Fuente: http://plos.srce.hr/



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