Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to SequencedReportar como inadecuado




Genome of Cnaphalocrocis medinalis Granulovirus, the First Crambidae-Infecting Betabaculovirus Isolated from Rice Leaffolder to Sequenced - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Cnaphalocrocis medinalis is a major pest of rice in South and South-East Asia. Insecticides are the major means farmers use for management. A naturally occurring baculovirus, C. medinalis granulovirus CnmeGV, has been isolated from the larvae and this has the potential for use as microbial agent. Here, we described the complete genome sequence of CnmeGV and compared it to other baculovirus genomes. The genome of CnmeGV is 112,060 base pairs in length, has a G+C content of 35.2%. It contains 133 putative open reading frames ORFs of at least 150 nucleotides. A hundred and one 101 of these ORFs are homologous to other baculovirus genes including 37 baculovirus core genes. Thirty-two 32 ORFs are unique to CnmeGV with no homologues detected in the GeneBank and 53 tandem repeats TRs with sequence length from 25 to 551 nt intersperse throughout the genome of CnmeGV. Six 6 homologous regions hrs were identified interspersed throughout the genome. Hr2 contains 11 imperfect palindromes and a high content of AT sequence about 73%. The unique ORF28 contains a coiled-coil region and a zinc finger-like domain of 4–50 residues specialized by two C2C2 zinc finger motifs that putatively bound two atoms of zinc. ORF21 encoding a chit-1 protein suggesting a horizontal gene transfer from alphabaculovirus. The putative protein presents two carbohydrate-binding module family 14 CBM 14 domains rather than other homologues detected from betabaculovirus that only contains one chit-binding region. Gene synteny maps showed the colinearity of sequenced betabaculovirus. Phylogenetic analysis indicated that CnmeGV grouped in the betabaculovirus, with a close relation to AdorGV. The cladogram obtained in this work grouped the 17 complete GV genomes in one monophyletic clade. CnmeGV represents a new crambidae host-isolated virus species from the genus Betabaculovirus and is most closely relative of AdorGV. The analyses and information derived from this study will provide a better understanding of the pathological symptoms caused by this virus and its potential use as a microbial pesticide.



Autor: Guangjie Han, Jian Xu , Qin Liu, Chuanming Li, Hongxing Xu, Zhongxian Lu

Fuente: http://plos.srce.hr/



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