Combined Administration of Taurine and Monoisoamyl Dmsa Protects Arsenic Induced Oxidative Injury in RatsReportar como inadecuado




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Oxidative Medicine and Cellular Longevity - Volume 1 2008, Issue 1, Pages 39-45

Department of Pharmacology and Toxicology, Defence Research and Development Establishment, Gwalior, India

Received 15 May 2008; Revised 3 June 2008; Accepted 16 June 2008

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Arsenic is a naturally occurring element that is ubiquitously present in the environment. High concentration of naturally occurring arsenic in drinking water is a major health problem in different parts of the world. Despite arsenic being a health hazard and a well documented carcinogen, no safe, effective and specific preventive or therapeutic measures are available. Among various recent strategies adopted, administration of an antioxidant has been reported to be the most effective. The present study was designed to evaluate the therapeutic efficacy of monoisoamyl dimercaptosuccinic acid MiADMSA, administered either individually or in combination with taurine post chronic arsenic exposure in rats. Arsenic exposed male rats 25 ppm, sodium arsenite in drinking water for 24 weeks were treated with taurine 100 mg-kg, i.p., once daily, monoisoamyl dimercaptosuccinic acid MiADMSA 50 mg-kg, oral, once daily either individually or in combination for 5 consecutive days. Biochemical variables indicative of oxidative stress along-with arsenic concentration in blood, liver and kidney were measured. Arsenic exposure significantly reduced blood δ-aminolevulinic acid dehydratase ALAD activity, a key enzyme involved in the heme biosynthesis and enhanced zinc protoporphyrin ZPP level. Clinical hematological variables like white blood cells WBC, mean cell hemoglobin MCH, and mean cell hemoglobin concentration MCHC showed significant decrease with a significant elevation in platelet PLT count. These changes were accompanied by significant decrease in superoxide dismutase SOD activity and increased catalase activity. Arsenic exposure caused a significant decrease in hepatic and renal glutathione GSH level and an increase in oxidized glutathione GSSG. These biochemical changes were correlated with an increased uptake of arsenic in blood, liver and kidney. Administration of taurine significantly reduced hepatic oxidative stress however co-administration of a higher dose of taurine 100 mg-kg and MiADMSA provided more pronounced effects in improving the antioxidant status of liver and kidney and reducing body arsenic burden compared to the individual treatment of MiADMSA or taurine. The results suggest that in order to achieve better effects of chelation therapy, co-administration of taurine with MiADMSA might be preferred.





Autor: Swaran J. S. Flora, Swapnila Chouhan, Gurusamy M. Kannan, Megha Mittal, and Harimohan Swarnkar

Fuente: https://www.hindawi.com/



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