Prevalence and Trends of Staphylococcus aureus Bacteraemia in Hospitalized Patients in South Africa, 2010 to 2012: Laboratory-Based Surveillance Mapping of Antimicrobial Resistance and Molecular EpidemiologyReportar como inadecuado




Prevalence and Trends of Staphylococcus aureus Bacteraemia in Hospitalized Patients in South Africa, 2010 to 2012: Laboratory-Based Surveillance Mapping of Antimicrobial Resistance and Molecular Epidemiology - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Introduction

We aimed to obtain an in-depth understanding on recent antimicrobial resistance trends and molecular epidemiology trends of S. aureus bacteraemia SAB.

Methods

Thirteen academic centres in South Africa were included from June 2010 until July 2012. S. aureus susceptibility testing was performed on the MicroScan Walkaway. Real-time PCR using the LightCycler 480 II was done for mecA and nuc. SCCmec and spa-typing were finalized with conventional PCR. We selected one isolate per common spa type per province for multilocus sequence typing MLST.

Results

S. aureus from 2709 patients were included, and 1231 46% were resistant to methicillin, with a significant decline over the three-year period p-value = 0.003. Geographical distribution of MRSA was significantly higher in Gauteng compared to the other provinces P<0.001. Children <5 years were significantly associated with MRSA with higher rates compared to all other age groups P = 0.01. The most prevalent SCCmec type was SCCmec type III 531 41% followed by type IV 402 31%. Spa-typing discovered 47 different spa-types. The five 87% most common spa-types were t037, t1257, t045, t064 and t012. Based on MLST, the commonest was ST612 clonal complex CC8 n = 7 followed by ST5 CC5 n = 4, ST36 CC30 n = 4 and ST239 CC8 n = 3.

Conclusions

MRSA rate is high in South Africa. Majority of the isolates were classified as SCCmec type III 41% and type IV 31%, which are typically associated with hospital and community- acquired infections, respectively. Overall, this study reveals the presence of a variety of hospital-acquired MRSA clones in South Africa dominance of few clones, spa 037 and 1257. Monitoring trends in resistance and molecular typing is recommended to detect changing epidemiological trends in AMR patterns of SAB.



Autor: Olga Perovic , Samantha Iyaloo, Ranmini Kularatne, Warren Lowman, Noma Bosman, Jeannette Wadula, Sharona Seetharam, Adriano Duse,

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados