Myocardial Ischemic Subject’s Thymus Fat: A Novel Source of Multipotent Stromal CellsReportar como inadecuado




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Objective

Adipose Tissue Stromal Cells ASCs have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue SAT is the most common source of these cells. The adult human thymus degenerates into adipose tissue TAT. However, it has never been studied before as a source of stem cells.

Material and Methods

We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects n = 32 according to the age of the subjects.

Results

TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs.

Conclusions

This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine.



Autor: Wilfredo Oliva-Olivera , Leticia Coín-Aragüez , Julián Salas, Said Lhamyani, Adriana-Mariel Gentile, Esteban Sarria García, A

Fuente: http://plos.srce.hr/



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