Identification of Candidate Genes Potentially Relevant to Chamber-Specific Remodeling in Postnatal Ventricular MyocardiumReportar como inadecuado




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Journal of Biomedicine and BiotechnologyVolume 2010 2010, Article ID 603159, 10 pages

Research ArticleDevelopmental Biology Unit, Institute of Health Sciences, University of La Coruña, Campus de Oza, As Xubias Street s-n, 15006 La Coruña, Spain

Received 8 October 2009; Accepted 7 January 2010

Academic Editor: Aikaterini Kontrogianni-Konstantopoulos

Copyright © 2010 Mario Torrado et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Molecular predisposition of postnatal ventricular myocardium to chamber-dependent concentric or eccentric remodeling remains largely elusive. To this end, we compared gene expression in the left LV versus right ventricle RV in newborn piglets, using a differential display reverse transcription-PCR DDRT-PCR technique. Out of more than 5600 DDRT-PCR bands, a total of 153 bands were identified as being differentially displayed. Of these, 96 bands were enriched in the LV, whereas the remaining 57 bands were predominant in the RV. The transcripts, displaying over twofold LV-RV expression differences, were sequenced and identified by BLAST comparison to known mRNA sequences. Among the genes, whose expression was not previously recognized as being chamber-dependent, we identified a small cohort of key regulators of muscle cell growth-proliferation MAP3K7IP2, MSTN, PHB2, APOBEC3F and gene expression PTPLAD1, JMJD1C, CEP290, which may be relevant to the chamber-dependent predisposition of ventricular myocardium to respond differentially to pressure LV and volume RV overloads after birth. In addition, our data demonstrate chamber-dependent alterations in expression of as yet uncharacterized novel genes, which may also be suitable candidates for association studies in animal models of LV-RV hypertrophy.





Autor: Mario Torrado, Raquel Iglesias, Beatriz Nespereira, and Alexander T. Mikhailov

Fuente: https://www.hindawi.com/



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