Supplementation with L-Glutamine and L-Alanyl-L-Glutamine Changes Biochemical Parameters and Jejunum Morphophysiology in Type 1 Diabetic Wistar RatsReportar como inadecuado




Supplementation with L-Glutamine and L-Alanyl-L-Glutamine Changes Biochemical Parameters and Jejunum Morphophysiology in Type 1 Diabetic Wistar Rats - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

We evaluated the effects of the supplementation with L-glutamine and glutamine dipeptide GDP on biochemical and morphophysiological parameters in streptozotocin-diabetic rats. For this purpose, thirty animals were distributed into six groups treated orally gavage during thirty days: non diabetic rats Control + saline, diabetic + saline; Control + L-glutamine 248 mg-kg, Diabetic + L-glutamine 248 mg-kg, Control + GDP 400 mg-kg, Diabetic + GDP 400 mg-kg. Diabetes was induced by an intravenous injection of streptozotocin 60 mg-kg and confirmed by fasting glucose ≥ 200 mg-dL. Physiological parameters, i.e., body mass, food intake, blood glucose, water intake, urine and faeces were evaluated during supplementation. After the period of supplementation, the animals were euthanized. The blood was collected for biochemical assays fructosamine, transaminases, lipid profile, total protein, urea, ammonia. Moreover, the jejunum was excised and stored for morphophysiological assays intestinal enzyme activity, intestinal wall morphology, crypt proliferative index, number of serotoninergic cells from the mucosa, and vipergic neurons from the submucosal tunica. The physiological parameters, protein metabolism and intestinal enzyme activity did not change with the supplementation with L-glutamine or GDP. In diabetic animals, transaminases and fructosamine improved with L-glutamine and GDP supplementations, while the lipid profile improved with L-glutamine. Furthermore, both forms of supplementation promoted changes in jejunal tunicas and wall morphometry of control and diabetic groups, but only L-glutamine promoted maintenance of serotoninergic cells and vipergic neurons populations. On the other hand, control animals showed changes that may indicate negative effects of L-glutamine. Thus, the supplementation with L-glutamine was more efficient for maintaining intestinal morphophysiology and the supplementation with GDP was more efficient to the organism as a whole. Thus, we can conclude that local differences in absorption and metabolism could explain the differences between the supplementation with L-glutamine or GDP.



Autor: Carlos Vinicius D. da Rosa, Silvia C. S. F. Azevedo, Roberto B. Bazotte, Rosane M. Peralta, Nilza C. Buttow, Maria Montserrat D.

Fuente: http://plos.srce.hr/



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