Comprehensive Survey of miRNA-mRNA Interactions Reveals That Ccr7 and Cd247 CD3 zeta are Posttranscriptionally Controlled in Pancreas Infiltrating T Lymphocytes of Non-Obese Diabetic NOD MiceReportar como inadecuado




Comprehensive Survey of miRNA-mRNA Interactions Reveals That Ccr7 and Cd247 CD3 zeta are Posttranscriptionally Controlled in Pancreas Infiltrating T Lymphocytes of Non-Obese Diabetic NOD Mice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

In autoimmune type 1 diabetes mellitus T1D, auto-reactive clones of CD4+ and CD8+ T lymphocytes in the periphery evolve into pancreas-infiltrating T lymphocytes PILs, which destroy insulin-producing beta-cells through inflammatory insulitis. Previously, we demonstrated that, during the development of T1D in non-obese diabetic NOD mice, a set of immune-inflammatory reactivity genes were differentially expressed in T lymphocytes. However, the posttranscriptional control involving miRNA interactions that occur during the evolution of thymocytes into PILs remains unknown. In this study, we postulated that miRNAs are differentially expressed during this period and that these miRNAs can interact with mRNAs involved in auto-reactivity during the progression of insulitis. To test this hypothesis, we used NOD mice to perform, for the first time, a comprehensive survey of miRNA and mRNA expression as thymocytes mature into peripheral CD3+ T lymphocytes and, subsequently, into PILs. Reconstruction of miRNA-mRNA interaction networks for target prediction revealed the participation of a large set of miRNAs that regulate mRNA targets related to apoptosis, cell adhesion, cellular regulation, cellular component organization, cellular processes, development and the immune system, among others. The interactions between miR-202-3p and the Ccr7 chemokine receptor mRNA or Cd247 Cd3 zeta chain mRNA found in PILs are highlighted because these interactions can contribute to a better understanding of how the lack of immune homeostasis and the emergence of autoimmunity e.g., T1D can be associated with the decreased activity of Ccr7 or Cd247, as previously observed in NOD mice. We demonstrate that these mRNAs are controlled at the posttranscriptional level in PILs.



Autor: Thais A. Fornari , Paula B. Donate , Amanda F. Assis , Claudia Macedo, Elza T. Sakamoto-Hojo, Eduardo A. Donadi, Geraldo A. Passo

Fuente: http://plos.srce.hr/



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