PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small RuminantsReport as inadecuate




PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small Ruminants - Download this document for free, or read online. Document in PDF available to download.

Journal of Parasitology Research - Volume 2014 2014, Article ID 294704, 6 pages -

Research Article

Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Departments of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Received 27 January 2014; Revised 18 March 2014; Accepted 19 March 2014; Published 24 April 2014

Academic Editor: C. Genchi

Copyright © 2014 Bijan Esmaeilnejad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to assess the prevalence of Babesia ovis infection in adult Rhipicephalus bursa and small ruminants in West Azerbaijan province, Iran. Blood samples were collected from 280 sheep and 122 goats of forty randomly selected flocks. Specific B. ovis fragment was detected in 67 animals 16.7%, of which 52 animals 18.6% were sheep and 15 animals 12.2% goats . Of the 848 R. bursa collected from naturally infested small ruminants and farm dogs, Babesia ovis was detected by PCR in salivary glands of 94 adult ticks. The frequency of B. ovis infection was higher in flocks with tick in comparison with animals without tick . Positive amplification from blood of ruminants, ticks, oviposition ticks, eggs, and larvae was subjected to restriction digestion with HphI. One RFLP profile was produced. The PCR-RFLP results indicated that one strain of B. ovis exists in this area. The results showed that the PCR was useful method to investigate the epidemiology of small ruminants’ babesiosis. Furthermore, R. Bursa, which can transovarially transmit B. ovis and as well as being widely distributed in West Azerbaijan province, Iran, might play an important role in the field as a natural vector of B. ovis.





Author: Bijan Esmaeilnejad, Mousa Tavassoli, Siamak Asri-Rezaei, Bahram Dalir-Naghadeh, Karim Mardani, Ghader Jalilzadeh-Amin, Mosta

Source: https://www.hindawi.com/



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