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BioMed Research InternationalVolume 2014 2014, Article ID 258409, 6 pages

Research Article

Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, 1st Affiliated Hospital of Guangzhou Medical University, No. 151 Yanjiang Road, Guangzhou 510120, China

Department of Orthopaedic Surgery, 1st People Hospital of Yunfu, No. 100 Construction of North Road, Yunfu 527300, China

Biomechanics & Medical Information Institute, Beijing University of Technology, No. 100 Flat Land Chaoyang District, Beijing 100022, China

Received 9 January 2014; Accepted 28 May 2014; Published 18 June 2014

Academic Editor: Guo-Xian Pei

Copyright © 2014 Yushu Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study was conducted in order to explore the mechanisms whereby parathyroid hormone PTH maintains in vitro proliferation of bone marrow mesenchymal stem cells BMSCs. Bone marrow was isolated from Sprague Dawley SD rat femurs, cultured in vitro, and passaged using a cell adherent culture method. The BMSC proliferation was evaluated by the methyl thiazolyl tetrazolium MTT assay and the fluorescence intensity of calcium ions in BMSCs was analyzed by laser scanning confocal microscopy LSCM. Our results show that BMSC proliferation in the experimental group treated with PTH was more significant than controls. The calcium ion fluorescence intensity in BMSCs was significantly higher for the experimental group as compared to the control group. For each group, there was significant difference in the fluorescence intensity of calcium ions in BMSCs between 7 d and 14 d. In conclusion, parathyroid hormone increased the fluorescence intensity of calcium ions in BMSCs, which might represent a key mechanism whereby BMSC proliferation is maintained.





Autor: Yushu Chen, Bo Bai, Shujiang Zhang, Jing Ye, Yi Chen, and Yanjun Zeng

Fuente: https://www.hindawi.com/



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