Optimization of Extraction and Enrichment of Steroidal Alkaloids from Bulbs of Cultivated Fritillaria cirrhosaReportar como inadecuado

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BioMed Research International - Volume 2014 2014, Article ID 258402, 15 pages -

Research ArticleDepartment of Pharmacognosy, West China College of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu 610041, China

Received 15 December 2013; Accepted 1 March 2014; Published 1 April 2014

Academic Editor: John J. Gildea

Copyright © 2014 Dongdong Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The bulbs of cultivated Fritillaria cirrhosa BCFC are used in China both for food and folk medicine due to its powerful biological activities. The aim of this study is to optimize the extraction and enrichment conditions of alkaloids from BCFC. Firstly, the orthogonal experimental design was used to optimize and evaluate four variables ethanol concentration, solid-liquid ratio, extraction time, and temperature. Thereafter, resin adsorption was as a means to enrich alkaloids. Among 16 tested resins, H-103 resin presented higher adsorption capacity and desorption ratio. The equilibrium experimental data of the adsorption of total alkaloids, imperialine, and peimisine were well-fitted to the pseudo-first-order kinetics model, Langmuir and Freundlich isotherms models. Finally, in order to optimize the parameters for purifying alkaloids, dynamic adsorption and desorption tests were carried out. After one run treatment with H-103 resin, the contents of total alkaloids, imperialine, and peimisine in the product were 21.40-, 18.31-, and 22.88-fold increased with recovery yields of 94.43%, 90.57%, and 96.16%, respectively.

Autor: Dongdong Wang, Shu Wang, Qingdan Du, Nanyi Wang, Simei Liu, Xiaoxia Wang, and Jinghui Jiang

Fuente: https://www.hindawi.com/


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