IMGT-HighV-QUEST Statistical Significance of IMGT Clonotype AA Diversity per Gene for Standardized Comparisons of Next Generation Sequencing Immunoprofiles of Immunoglobulins and T Cell ReceptorsReportar como inadecuado




IMGT-HighV-QUEST Statistical Significance of IMGT Clonotype AA Diversity per Gene for Standardized Comparisons of Next Generation Sequencing Immunoprofiles of Immunoglobulins and T Cell Receptors - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The adaptive immune responses of humans and of other jawed vertebrate species gnasthostomata are characterized by the B and T cells and their specific antigen receptors, the immunoglobulins IG or antibodies and the T cell receptors TR up to 2.1012 different IG and TR per individual. IMGT, the international ImMunoGeneTics information system http:-www.imgt.org, was created in 1989 by Marie-Paule Lefranc Montpellier University and CNRS to manage the huge and complex diversity of these antigen receptors. IMGT built on IMGT-ONTOLOGY concepts of identification keywords, description labels, classification gene and allele nomenclature and numerotation IMGT unique numbering, is at the origin of immunoinformatics, a science at the interface between immunogenetics and bioinformatics. IMGT-HighV-QUEST, the first web portal, and so far the only one, for the next generation sequencing NGS analysis of IG and TR, is the paradigm for immune repertoire standardized outputs and immunoprofiles of the adaptive immune responses. It provides the identification of the variable V, diversity D and joining J genes and alleles, analysis of the V-D-J junction and complementarity determining region 3 CDR3 and the characterization of the ‘IMGT clonotype AA’ AA for amino acid diversity and expression. IMGT-HighV-QUEST compares outputs of different batches, up to one million nucleotide sequencesfor the statistical module. These high throughput IG and TR repertoire immunoprofiles are of prime importance in vaccination, cancer, infectious diseases, autoimmunity and lymphoproliferative disorders, however their comparative statistical analysis still remains a challenge. We present a standardized statistical procedure to analyze IMGT-HighV-QUEST outputs for the evaluation of the significance of the IMGT clonotype AA diversity differences in proportions, per gene of a given group, between NGS IG and TR repertoire immunoprofiles. The procedure is generic and suitable for evaluating significance of the IMGT clonotype AA diversity and expression per gene, and for any IG and TR immunoprofiles of any species.



Autor: Safa Aouinti, Dhafer Malouche, Véronique Giudicelli, Sofia Kossida , Marie-Paule Lefranc

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados