Inositol Polyphosphate-4-Phosphatase Type I Negatively Regulates Phagocytosis via Dephosphorylation of Phagosomal PtdIns3,4P2Report as inadecuate




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Phagocytosis is a highly conserved process whereby phagocytic cells engulf pathogens and apoptotic bodies. The present study focused on the role of inositol polyphosphate-4-phosphatase type I Inpp4a in phagocytosis. Raw264.7 cells that express shRNA against Inpp4a shInpp4a cells showed significantly increased phagocytic activity. The introduction of shRNA-resistant human Inpp4a abolished this increase. Macrophages from Inpp4a knockout mice showed similar increases in the phagocytic activity. Inpp4a was recruited to the phagosome membrane by a mechanism other than the direct interaction with Rab5. PtdIns3,4P2 increased on the phagosome of shInpp4a cells, while PtdIns3P significantly decreased. The results indicate that Inpp4a negatively regulates the phagocytic activity of macrophages as a member of the sequential dephosphorylation system that metabolizes phagosomal PtdIns3,4,5P3 to PtdIns3P.



Author: Kiyomi Nigorikawa, Kaoru Hazeki , Junko Sasaki, Yumio Omori, Mikiko Miyake, Shin Morioka, Ying Guo, Takehiko Sasaki, Osamu Hazeki

Source: http://plos.srce.hr/



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