A Comparison of Ligase Chain Reaction to Polymerase Chain Reaction in the Detection of Chlamydia trachomatis Endocervical InfectionsReport as inadecuate




A Comparison of Ligase Chain Reaction to Polymerase Chain Reaction in the Detection of Chlamydia trachomatis Endocervical Infections - Download this document for free, or read online. Document in PDF available to download.

Infectious Diseases in Obstetrics and Gynecology - Volume 6 1998, Issue 2, Pages 57-60

Research Article

Division of Gynecology, Department of Obstetrics and Gynecology, University of Florida College of Medicine, PO BoX 100294, Room M-302E, Gainesville, FL 32610-0294, USA

Department of Emergency Medicine, University of Florida College of Medicine, Gainesville, FL, USA

Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA

Received 6 February 1998; Accepted 5 May 1998

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective: To compare the reliability of ligase chain reaction LCR to polymerase chain reaction PCR in detecting Chlamydia trachomatis endocervical infections.

Methods: We conducted a prospective study of 486 patients at risk for chlamydial infection of theendocervix. We obtained two endocervical specimens from each patient and used LCR and PCR todetect C. trachomatis. Discrepant results between the two techniques were resolved by repeat testingand by testing for the major outer membrane protein MOMP gene, if necessary. We determinedthe sensitivity, specificity, positive predictive value, and negative predictive value for each test, usingconcordant results or MOMP gene results as the -gold standard-.

Results: Of the 486 patients, 42 8.6% had evidence of C. trachomatis infection after resolution ofdiscrepant results. Of the 42 true positive specimens, 41 were positive by initial LCR and 38 werepositive by initial PCR. Of the 444 true negative specimens, none had a positive initial LCR result,while 2 had a positive initial PCR test. Therefore, compared to the gold standard, LCR had asensitivity of 97.6% and specificity of 100%, while PCR had a sensitivity of 90% and a specificity of99.5%. The positive and negative predictive values of LCR were 100% and 99.8%, respectively.PCR had a positive predictive value of 95% and a negative predictive value of 99.1%. The differencein sensitivity of LCR versus PCR was not statistically significant P = .125.

Conclusion: LCR and PCR perform equally well in detecting C. trachomatis endocervical infections.





Author: J. D. Davis, P. K. Riley, C. W. Peters, and K. H. Rand

Source: https://www.hindawi.com/



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