Reinstatement of Morphine-Induced Conditioned Place Preference in Mice by Priming InjectionsReport as inadecuate

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Neural Plasticity - Volume 10 2003, Issue 4, Pages 279-290

Departamento de Psicobiología, Facultad de Psicología, Universitat de Valencia, Avda. Blasco Ibáñez, Valencia 21. 46010, Spain

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


To construct a model of relapse of drugabuse in mice, the induction, we evaluated theextinction and reinstatement of morphine-inducedplace preference. In Experiment 1, weexamined the effects of morphine 0, 2, 3, 5, 10,20 and 40 mg-kg in the conditioned placepreference CPP paradigm. Mice showed CPPwith 5, 10, 20 and 40 mg-kg. In Experiment 2,we evaluated the effects of two different extinctionprocedures. After conditioning with 40mg-kg of morphine, the mice underwent dailyextinction sessions of 60 or 15 min of duration.CPP was extinguished after seven and ninesessions, respectively. In Experiment 3, wetested the reinstating effects of several primingdoses of morphine. Mice were conditioned with40 mg-kg of morphine and underwent the daily15 min extinction sessions until CPP was nolonger evident. Then, the effects of morphine 0,2, 3, 5, 10, 20, 40 mg-kg, i.p. were evaluated.CPP was reinstated by doses from 5 mg-kgupward. The results show that morphine priminginjections are effective in reactivating opiateseekingbehavior in mice, and thus, the CPPparadigm might be useful to investigate themechanisms underlying relapse of drug abuse.

Author: B. Ribeiro Do Couto, M. A. Aguilar, C. Manzanedo, M. Rodríguez-Arias, and J. Miñarro



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