Improvement of Starch Digestion Using α-Amylase Entrapped in Pectin-Polyvinyl Alcohol BlendReport as inadecuate




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BioMed Research International - Volume 2015 2015, Article ID 145903, 9 pages -

Research Article

Departamento de Química, Instituto Federal de Educação Ciência e Tecnologia de Goiás, 76400-000 Uruaçu, GO, Brazil

Departamento de Bioquímica e Biologia Molecular, Laboratório de Química de Polímeros, Universidade Federal de Goiás, Caixa Postal 131, 74001-970 Goiânia, GO, Brazil

Departamento de Ciências Fisiológicas, Laboratório de Fisiologia da Digestão, Universidade Federal de Goiás, Caixa Postal 131, 74001-970 Goiânia, GO, Brazil

Laboratório de Biotecnologia, Unidade Universitária de Ciências Exatas e Tecnológicas, Universidade Estadual de Goiás, 75.132-903 Anápolis, GO, Brazil

Received 22 December 2014; Revised 21 March 2015; Accepted 22 March 2015

Academic Editor: Zheng Guo

Copyright © 2015 Maurício Cruz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Polyvinyl alcohol PVA and pectin blends were used to entrap α-amylase Termamyl using glutaraldehyde as a cross-linker. The effect of glutaraldehyde concentration 0.25, 0.5, 0.75, 1.0, and 1.25% on the activity of the immobilized enzyme and rate of enzyme released was tested during a 24 h period. Characteristics of the material, such as scanning electron microscopy SEM, tensile strength TS, elongation, and rate of dissolution in water pH 5.7, ruminal buffering solution pH 7.0, and reactor containing 0.1 mol L

sodium phosphate buffer pH 6.5, were also analyzed. SEM results showed that the surfaces of the pectin-PVA-amylase films were highly irregular and rough. TS values increased as a function of glutaraldehyde concentration, whereas percentage of elongation %E decreased. Pectin-PVA-amylase films presented similar values of solubility in the tested solvents. The material obtained with 0.25% glutaraldehyde performed best with repeated use active for 24 h, in a phosphate buffer reactor. By contrast, the material obtained with 1.25% glutaraldehyde presented higher performance during in vitro testing using an artificial rumen. The results suggest that pectin-PVA-amylase is a highly promising material for biotechnological applications.





Author: Maurício Cruz, Kátia Fernandes, Cristine Cysneiros, Reginaldo Nassar, and Samantha Caramori

Source: https://www.hindawi.com/



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