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International Journal of DentistryVolume 2011 2011, Article ID 435830, 6 pages

Research ArticleDepartment of Operative Dentistry and Periodontology, Center of Dentistry, Oral Medicine, and Maxillofacial Surgery, Eberhard-Karls University Tübingen, 72076 Tübingen, Germany

Received 24 June 2011; Accepted 16 August 2011

Academic Editor: Alexandre Rezende Vieira

Copyright © 2011 Eva-Maria Decker et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Caries development in the presence of Streptococcus mutans is associated not only with the production of extracellular water-insoluble polymers but also is based on water-soluble polysaccharides. The aim of this study was the evaluation of a novel glucan-specific Lectin assay for monitoring water-soluble EPS produced by S. mutans during several growth periods in different media. S. mutans cultures were grown for 24 h, 48 h, and 144 h in medium deficient of sucrose A and medium supplemented with 5% sucrose B. Microtiter well plates were coated with cell-free supernatants followed by the addition of labeled Concanavalin-A and enzyme substrate. The substrate reactions were kinetically detected at 405 nm. The validation of the assay was performed using carbohydrates dextran, xanthan, and sucrose as reference. This new Concanavalin-A-based assay showed the highest sensitivity for dextran and revealed that the glucan production of S. mutans reached its maximum at 144 h in medium B according to bacterial maturation.





Autor: Eva-Maria Decker, Ilka Dietrich, Christian Klein, and Christiane von Ohle

Fuente: https://www.hindawi.com/



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