The Effect of Primary Fixation with Standard Postfixation and the Duration of Hydrolysis on Nuclear Features in Image CytometryReportar como inadecuado




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Analytical Cellular Pathology - Volume 17 1998, Issue 3, Pages 131-144



Department Cytopathology, Institute of Oncology, 1000 Ljubljana, Slovenia

Oncometrics Imaging Corp., Vancouver, BC, Canada V5Z 1L3

Received 16 December 1997; Accepted 15 October 1998

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effect of three primary fixation procedures, used in the preparation of routine cytological samples: air-drying, Delaunay, and Saccomanno fixation, with postfixation in modified Böhm–Sprenger fixative, on nuclear features as a function of hydrolysis time is reported. Three different cell types: lymphatic cells tonsil, epithelial cells buccal mucosa and mesenchymal cells uterine myometrium were used for the study. Our findings show, that generally not all features have the same plateau times as the IOD integrated optical density, and that many features show different values depending on cell type and fixation method. It is therefore recommended that for any primary fixative used in routine clinical work and for each cell type, the hydrolysis curve for all nuclear features to be used in sample analysis should be established.





Autor: Margareta Fležar, Alexei Doudkine, and Marija Us-Krašovec

Fuente: https://www.hindawi.com/



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