Microclonal Multiplication of wild Cherry Prunus avium L. from Shoot Tips and Root Sucker BudsReportar como inadecuado




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Acta Botanica Croatica, Vol.53 No.1 December 1994. -

The effects of different combinations and concentrations of the growth regulators: 6-benzylaminopurine BA, 6-furfurylaminopurine KIN, N6- 2-isopentenyl adenine 2iP, indole-3-butyric acid IBA, indole-3-acetic acid IAA and a-naphthaleneacetic acid NAA on axillary shoot multiplication rates for wild cherry Prunus avium L. shoot explants were determined. Apical shoot tips and axillary buds from juvenile trees 5-year old and from root suckers of mature trees 55-year old were used as initial explants for establishment of multiple shoot cultures. Factorial tests for growth regulator interactions with the two genotypes showed significant differences in shoot multiplication rate with numerous auxin- and cytokinin-amended basal media. For genotype P5, the most effective growth regulator combination was 5.0 mg-L KIN and 1.0 mg-L IAA. Genotype 5-11 responded best to 1.0 mg-L BA and 5.0 mg-L IBA. Both clones multiplied easily in culture, and shoot multiplication of the mature genotype was not inhibited.



Autor: Branka Pevalek-Kozlina - ; Hrvatska Charles H. Michler - ; USA Sibila Jelaska - ; Hrvatska

Fuente: http://hrcak.srce.hr/



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