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Journal of Biomedicine and BiotechnologyVolume 2009 2009, Article ID 769568, 10 pages

Research Article

UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, University Putra Malaysia, Serdang, 43400 Selangor, Malaysia

Department of Clinical Biochemistry, Faculty of Medicine and Health Sciences, University of Sana'a, Sana'a, Yemen

Received 7 August 2008; Revised 5 December 2008; Accepted 7 January 2009

Academic Editor: Yan Luo

Copyright © 2009 Siddig Ibrahim Abdel Wahab et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Zerumbone ZER, a potential anticancer compound, isolated from the fresh rhizomes of Zingiber zerumbet. In this investigation, the cytotoxic properties of ZER were evaluated, on cancer cells of human cervix HeLa, breast and ovary, and normal cells of Chinese Hamster ovary, using MTT assay. Apoptogenic effects of ZER on HeLa were studied using fluorescence microscopy AO-PI double staining, scanning and transmission electron microscopy SEM and TEM, and colorimetric assay of the apoptosis promoter enzyme, caspase-3. The results of MTT assay showed that ZER has less effect on normal cells compared to cancer cells. The lowest of ZER was observed on HeLa cells. Cytological observations showed nuclear and chromatin condensation, cell shrinkage, multinucleation, abnormalities of mitochondrial cristae, membrane blebbing, holes, cytoplasmic extrusions and formation of apoptotic bodies as confirmed collectively by double staining of AO-PI, SEM and TEM. Statistical analysis two-tailed t-test of differential counting of 200 cells under fluorescence microscope revealed significant difference in apoptotic cells populations between treated and untreated HeLa cells. In addition, ZER has increased the cellular level of caspase-3 on the treated HeLa cells. It could be concluded that ZER was able to produce distinctive morphological features of cell death that corresponds to apoptosis.

Autor: Siddig Ibrahim Abdel Wahab, Ahmad Bustamam Abdul, Adel Sharaf Alzubairi, Manal Mohamed Elhassan, and Syam Mohan



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