Proteomic Alterations in B Lymphocytes of Sensitized Mice in a Model of Chemical-Induced AsthmaReportar como inadecuado




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Introduction and Aim

The role of B-lymphocytes in chemical-induced asthma is largely unknown. Recent work demonstrated that transferring B lymphocytes from toluene diisocyanate TDI-sensitized mice into naïve mice, B cell KO mice and SCID mice, triggered an asthma-like response in these mice after a subsequent TDI-challenge. We applied two-dimensional difference gel electrophoresis 2D-DIGE to describe the -sensitized signature- of B lymphocytes comparing TDI-sensitized mice with control mice.

Results

Sixteen proteins were identified that were significantly up- or down-regulated in B lymphocytes of sensitized mice. Particularly differences in the expression of cyclophilin A, cofilin 1 and zinc finger containing CCHC domain protein 11 could be correlated to the function of B lymphocytes as initiators of T lymphocyte independent asthma-like responses.

Conclusion

This study revealed important alterations in the proteome of sensitized B cells in a mouse model of chemical-induced asthma, which will have an important impact on the B cell function.



Autor: Steven Haenen , Jeroen A. J. Vanoirbeek , Vanessa De Vooght, Liliane Schoofs, Benoit Nemery, Elke Clynen , Peter H. M. Hoet

Fuente: http://plos.srce.hr/



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