Circulating MicroRNAs Characterizing Patients with Insufficient Coronary Collateral Artery FunctionReportar como inadecuado




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Background

Coronary collateral arteries function as natural bypasses in the event of coronary obstruction. The degree of collateral network development significantly impacts the outcome of patients after an acute myocardial infarction AMI. MicroRNAs miRNAs, miRs have arisen as biomarkers to identify heterogeneous patients, as well as new therapeutic targets in cardiovascular disease. We sought to identify miRNAs that are differentially expressed in chronic total occlusion CTO patients with well or poorly developed collateral arteries.

Methods and Results

Forty-one CTO patients undergoing coronary angiography and invasive assessment of their coronary collateralization were dichotomized based on their collateral flow index CFI. After miRNA profiling was conducted on aortic plasma, four miRNAs were selected for validation by real-time quantitative reverse transcription polymerase chain reaction in patients with low CFI<0.39 and high CFI>0.39 collateral artery capacity. We confirmed significantly elevated levels of miR423-5p p<0.05, miR10b p<0.05, miR30d p<0.05 and miR126 p<0.001 in patients with insufficient collateral network development. We further demonstrated that each of these miRNAs could serve as circulating biomarkers to discriminate patients with low collateral capacity p<0.01 for each miRNA. We also determined significantly greater expression of miR30d p<0.05 and miR126 p<0.001 in CTO patients relative to healthy controls.

Conclusion

The present study identifies differentially expressed miRNAs in patients with high versus low coronary collateral capacity. We have shown that these miRNAs can function as circulating biomarkers to discriminate between patients with insufficient or sufficient collateralization. This is the first study to identify miRNAs linked to coronary collateral vessel function in humans.



Autor: Nazanin Hakimzadeh, A. Yaël Nossent, Anja M. van der Laan, Stephan H. Schirmer, Maurice W. J. de Ronde, Sara-Joan Pinto-Sietsma,

Fuente: http://plos.srce.hr/



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