Changes in Diffusion Kurtosis Imaging and Magnetic Resonance Spectroscopy in a Direct Cranial Blast Traumatic Brain Injury dc-bTBI ModelReportar como inadecuado




Changes in Diffusion Kurtosis Imaging and Magnetic Resonance Spectroscopy in a Direct Cranial Blast Traumatic Brain Injury dc-bTBI Model - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Explosive blast-related injuries are one of the hallmark injuries of veterans returning from recent wars, but the effects of a blast overpressure on the brain are poorly understood. In this study, we used in vivo diffusion kurtosis imaging DKI and proton magnetic resonance spectroscopy MRS to investigate tissue microstructure and metabolic changes in a novel, direct cranial blast traumatic brain injury dc-bTBI rat model. Imaging was performed on rats before injury and 1, 7, 14 and 28 days after blast exposure ~517 kPa peak overpressure to the dorsum of the head. No brain parenchyma abnormalities were visible on conventional T2-weighted MRI, but microstructural and metabolic changes were observed with DKI and proton MRS, respectively. Increased mean kurtosis, which peaked at 21 days post injury, was observed in the hippocampus and the internal capsule. Concomitant increases in myo-Inositol Ins and Taurine Tau were also observed in the hippocampus, while early changes at 1 day in the Glutamine Gln were observed in the internal capsule, all indicating glial abnormality in these regions. Neurofunctional testing on a separate but similarly treated group of rats showed early disturbances in vestibulomotor functions days 1–14, which were associated with imaging changes in the internal capsule. Delayed impairments in spatial memory and in rapid learning, as assessed by Morris Water Maze paradigms days 14–19, were associated with delayed changes in the hippocampus. Significant microglial activation and neurodegeneration were observed at 28 days in the hippocampus. Overall, our findings indicate delayed neurofunctional and pathological abnormalities following dc-bTBI that are silent on conventional T2-weighted imaging, but are detectable using DKI and proton MRS.



Autor: Jiachen Zhuo, Kaspar Keledjian, Su Xu, Adam Pampori, Volodymyr Gerzanich, J. Marc Simard , Rao P. Gullapalli

Fuente: http://plos.srce.hr/



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