Royal Jelly Prevents Osteoporosis in Rats: Beneficial Effects in Ovariectomy Model and in Bone Tissue Culture ModelReportar como inadecuado

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Evidence-Based Complementary and Alternative Medicine - Volume 3 2006, Issue 3, Pages 339-348

Original Article

Department of Dental Hygiene, Fukuoka College of Health Sciences, Fukuoka, Japan

Department of Dental Engineering, Section of Bioengineering, Fukuoka Dental College, Fukuoka, Japan

Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Shizuoka, Japan

Institute for Health Science, Yamada Apiculture Center Inc., Okayama, Japan

Philadelphia Biomedical Research Institute, King of Prussia, PA, USA

Received 15 March 2006; Accepted 16 March 2006

Copyright © 2006 Saburo Hidaka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Royal jelly RJ has been used worldwide for many years as medical products, health foods and cosmetics. Since RJ contains testosterone and has steroid hormone-type activities, we hypothesized that it may have beneficial effects on osteoporosis. We used both an ovariectomized rat model and a tissue culture model. Rats were divided into eight groups as follows: sham-operated Sham, ovariectomized OVX, OVX given 0.5% w-w raw RJ, OVX given 2.0% w-w RJ, OVX given 0.5% w-w protease-treated RJ pRJ, OVX given 2.0% w-w pRJ, OVX given 17β-estradiol and OVX given its vehicle, respectively. The Ovariectomy decreased tibial bone mineral density BMD by 24%. Administration of 17β-estradiol to OVX rats recovered the tibial BMD decrease by 100%. Administration of 2.0% w-w RJ and 0.5–2.0% w-w pRJ to OVX rats recovered it by 85% or more. These results indicate that both RJ and pRJ are almost as effective as 17β-estradiol in preventing the development of bone loss induced by ovariectomy in rats. In tissue culture models, both RJ and pRJ increased calcium contents in femoral-diaphyseal and femoral-metaphyseal tissue cultures obtained from normal male rats. However, in a mouse marrow culture model, they neither inhibited the parathyroid hormone PTH-induced calcium loss nor affected the formation of osteoclast-like cells induced by PTH in mouse marrow culture system. Therefore, our results suggest that both RJ and pRJ may prevent osteoporosis by enhancing intestinal calcium absorption, but not by directly antagonizing the action of PTH.

Autor: Saburo Hidaka, Yoshizo Okamoto, Satoshi Uchiyama, Akira Nakatsuma, Ken Hashimoto, Tsuyoshi Ohnishi, and Masayoshi Yamaguchi



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