A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2Reportar como inadecuado




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Acta Pharmaceutica, Vol.66 No.3 September 2016. -

Silicon-substituted calcium phosphate Si-CaP was developed in our laboratory as a biomaterial for delivery in bone tissue engineering. It was fabricated as a 3D-construct of scaffolds using chitosan-trisodium polyphosphate TPP cross-linked networks. In this study, heparin was covalently bonded to the residual -NH2 groups of chitosan on the scaffold applying carbodiimide chemistry. Bonded heparin was not leached away from scaffold surfaces upon vigorous washing or extended storage. Recombinant human bone morphogenetic protein 2 rhBMP-2 was bound to conjugated scaffolds by ionic interactions between the negatively charged SO42 clusters of heparin and positively charged amino acids of rhBMP-2. The resulting scaffolds were inspected for bone regenerative capacity by subcutaneous implanting in rats. Histological observation and mineralization assay were performed after 4 weeks of implantation. Results from both in vitro and in vivo experiments suggest the potential of the developed scaffolds for bone tissue engineering applications in the future.

heparin; carbodiimide; rhBMP2; delivery scaffold; bone tissue engineering



Autor: SUPHANNEE THANYAPHOO - ; Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University

Fuente: http://hrcak.srce.hr/



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