Genome-Wide Mapping of 5mC and 5hmC Identified Differentially Modified Genomic Regions in Late-Onset Severe Preeclampsia: A Pilot StudyReportar como inadecuado




Genome-Wide Mapping of 5mC and 5hmC Identified Differentially Modified Genomic Regions in Late-Onset Severe Preeclampsia: A Pilot Study - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Preeclampsia PE is a leading cause of perinatal morbidity and mortality. However, as a common form of PE, the etiology of late-onset PE is elusive. We analyzed 5-methylcytosine 5mC and 5-hydroxymethylcytosine 5hmC levels in the placentas of late-onset severe PE patients n = 4 and normal controls n = 4 using a hydroxymethylated DNA immunoprecipitation approach combined with deep sequencing hMeDIP-seq, and the results were verified by hMeDIP-qPCR. The most significant differentially methylated regions DMRs were verified by MassARRAY EppiTYPER in an enlarged sample size n = 20. Bioinformatics analysis identified 714 peaks of 5mC that were associated with 403 genes and 119 peaks of 5hmC that were associated with 61 genes, thus showing significant differences between the PE patients and the controls >2-fold, p<0.05. Further, only one gene, PTPRN2, had both 5mC and 5hmC changes in patients. The ErbB signaling pathway was enriched in those 403 genes that had significantly different5mC level between the groups. This genome-wide mapping of 5mC and 5hmC in late-onset severe PE and normal controls demonstrates that both 5mC and 5hmC play epigenetic roles in the regulation of the disease, but work independently. We reveal the genome-wide mapping of DNA methylation and DNA hydroxymethylation in late-onset PE placentas for the first time, and the identified ErbB signaling pathway and the gene PTPRN2 may be relevant to the epigenetic pathogenesis of late-onset PE.



Autor: Lisha Zhu, Ruitu Lv, Lingchun Kong, Haidong Cheng , Fei Lan , Xiaotian Li

Fuente: http://plos.srce.hr/



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