Opposing Effects of Omega-3 and Omega-6 Long Chain Polyunsaturated Fatty Acids on the Expression of Lipogenic Genes in Omental and Retroperitoneal Adipose Depots in the RatReportar como inadecuado




Opposing Effects of Omega-3 and Omega-6 Long Chain Polyunsaturated Fatty Acids on the Expression of Lipogenic Genes in Omental and Retroperitoneal Adipose Depots in the Rat - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Nutrition and MetabolismVolume 2010 2010, Article ID 927836, 9 pages

Research Article

FOODplus Research Centre, School of Agriculture, Food and Wine, The University of Adelaide, Adelaide 5064, Australia

Division of Health Sciences, Sansom Institute for Health Research, University of South Australia, Adelaide SA 5001, Australia

Rheumatology Unit, School of Medicine, The University of Adelaide, Adelaide SA 5005, Australia

Received 10 November 2009; Revised 25 May 2010; Accepted 23 June 2010

Academic Editor: Michael M. Müller

Copyright © 2010 B. S. Muhlhausler et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to determine the effect of varying dietary intake of the major n-3 PUFA in human diets, -linolenic acid ALA; 18 : 3n-3, on expression of lipogenic genes in adipose tissue. Rats were fed diets containing from 0.095%en to 6.3%en ALA and a constant n-6 PUFA level for 3 weeks. Samples from distinct adipose depots omental and retroperitoneal were collected and mRNA expression of the pro-lipogenic transcription factors Sterol-Retinoid-Element-Binding-Protein1c SREBP1c and Peroxisome Proliferator Activated Receptor- PPAR , lipogenic enzymes Sterol-coenzyme Desaturase1 SCD-1, Fatty Acid Synthase FAS, lipoprotein lipase LPL and glycerol-3-phosphate dehydrogenase G3PDH and adipokines leptin and adiponectin determined by qRT-PCR. Increasing dietary ALA content resulted in altered expression of SREBP1c, FAS and G3PDH mRNA in both adipose depots. SREBP1c mRNA expression was related directly to n-6 PUFA concentrations omental, ; ; Retroperitoneal, ; , and inversely to n-3 PUFA concentrations omental, ; ; Retroperitoneal, ; independent of diet. The relationship between total n-6 PUFA and SREBP1c mRNA expression persisted when the effects of n-3 PUFA were controlled for. Altering red blood cell concentrations of n-3 PUFA is thus associated with altered expression of lipogenic genes in a depot-specific manner and this effect is modulated by prevailing n-6 PUFA concentrations.





Autor: B. S. Muhlhausler, R. Cook-Johnson, M. James, D. Miljkovic, E. Duthoit, and R. Gibson

Fuente: https://www.hindawi.com/



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