Corneal Stroma Regeneration with Acellular Corneal Stroma Sheets and Keratocytes in a Rabbit ModelReportar como inadecuado




Corneal Stroma Regeneration with Acellular Corneal Stroma Sheets and Keratocytes in a Rabbit Model - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Acellular corneal stroma matrix has been used for corneal stroma engineering. However, because of its compact tissue structure, regrowth of keratocytes into the scaffold is difficult. Previously, we developed a sandwich model for cartilage engineering using acellular cartilage sheets. In the present study, we tested this model for corneal stroma regeneration using acellular porcine corneal stroma APCS sheets and keratocytes. Porcine corneas were decellularized by NaCl treatment, and the APCS was cut into 20-μm-thick sheets. A rabbit corneal stroma defect model was created by lamellar keratoplasty and repaired by transplantation of five pieces of APCS sheets with keratocytes. Six months after transplantation, transparent corneas were present in the experimental group, which were confirmed by anterior segment optical coherence tomography examination and transmittance examination. The biomechanical properties in the experimental group were similar to those of normal cornea. Histological analyses showed an even distribution of keratocytes and well-oriented matrix in the stroma layer in the experimental group. Together, these results demonstrated that the sandwich model using acellular corneal stroma sheets and keratocytes could be potentially useful for corneal stroma regeneration.



Autor: Xiao Yun Ma, Yun Zhang, Dan Zhu, Yang Lu, Guangdong Zhou, Wei Liu, Yilin Cao , Wen Jie Zhang

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados