Cell Population Kinetics of Collagen Scaffolds in Ex Vivo Oral Wound RepairReportar como inadecuado

Cell Population Kinetics of Collagen Scaffolds in Ex Vivo Oral Wound Repair - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Biodegradable collagen scaffolds are used clinically for oral soft tissue augmentation to support wound healing. This study sought to provide a novel ex vivo model for analyzing healing kinetics and gene expression of primary human gingival fibroblasts hGF within collagen scaffolds. Sponge type and gel type scaffolds with and without platelet-derived growth factor-BB PDGF were assessed in an hGF containing matrix. Morphology was evaluated with scanning electron microscopy, and hGF metabolic activity using MTT. We quantitated the population kinetics within the scaffolds based on cell density and distance from the scaffold border of DiI-labled hGFs over a two-week observation period. Gene expression was evaluated with gene array and qPCR. The sponge type scaffolds showed a porous morphology. Absolute cell number and distance was higher in sponge type scaffolds when compared to gel type scaffolds, in particular during the first week of observation. PDGF incorporated scaffolds increased cell numbers, distance, and formazan formation in the MTT assay. Gene expression dynamics revealed the induction of key genes associated with the generation of oral tissue. DKK1, CYR61, CTGF, TGFBR1 levels were increased and integrin ITGA2 levels were decreased in the sponge type scaffolds compared to the gel type scaffold. The results suggest that this novel model of oral wound healing provides insights into population kinetics and gene expression dynamics of biodegradable scaffolds.

Autor: Hermann Agis, Amy Collins, Andrei D. Taut, Qiming Jin, Laura Kruger, Christoph Görlach, William V. Giannobile

Fuente: http://plos.srce.hr/


Documentos relacionados