Serological and molecular detection of spotted fever group Rickettsia in a group of pet dogs from Luanda, AngolaReportar como inadecuado

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Parasites and Vectors

, 10:271

12th Symposium on Companion Vector-borne Diseases CVBD


BackgroundInfections with tick-borne rickettsiae can cause diseases well known in humans but still not so well characterized in dogs. Susceptibility to infection depends on the virulence of Rickettsia spp. and only a few of them have been described to cause disease in dogs. The aim of this study was to investigate the exposure to Rickettsia spp. among a group of pet dogs from Luanda, Angola.

ResultsOut of 103 dogs included in the study, 62 60.2% were infested with ticks. Plasma specimens tested for serology by an immunofluorescence assay IFA revealed that six 5.8% dogs had detectable immunoglobulin G IgG antibodies to spotted fever group Rickettsia SFGR, with endpoint titers of 64 for two dogs, 128 for three dogs and 1024 for one dog. From the seropositive group of dogs, five 83% of them were males, with their age ranging from 1 to 8 years old. Among the seropositive dogs, four 66.7% were parasitized with ticks and no breed or cross was found to be associated with specific antibodies. Rickettsia spp. DNA was detected by nested-polymerase chain reaction PCR in two 1.9% dogs that were found to be seronegative.

ConclusionsSeroprevalence and molecular detection of Rickettsia spp. infection in this group of pet dogs from Luanda is low compared with other studies performed in the same type of hosts in other areas. Although many dogs were parasitized with ticks, a low prevalence of Rickettsia spp. could be related with the hypothesis of a low rickettsial prevalence in the infesting ticks. This study provides evidence that dogs in Luanda are exposed to Rickettsia spp., but further studies are needed to better characterize the bacterial infections in dogs and in their ectoparasites.

KeywordsAngola Dogs Rickettsia Seroprevalence Spotted fever group AbbreviationsCVBDCanine vector-borne diseases

IFAImmunofluorescence assay

IgGImmunoglobulin G

PCRPolymerase chain reaction

SFGRSpotted fever group Rickettsia

Autor: Patrícia F. Barradas - Hugo Vilhena - Ana Cristina Oliveira - Sara Granada - Irina Amorim - Paula Ferreira - Luís Cardo


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