Production, purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juiceReportar como inadecuado




Production, purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

3 Biotech

, 7:122

First Online: 31 May 2017Received: 03 December 2016Accepted: 02 February 2017DOI: 10.1007-s13205-017-0760-3

Cite this article as: Anand, G., Yadav, S. & Yadav, D. 3 Biotech 2017 7: 122. doi:10.1007-s13205-017-0760-3

Abstract

Polygalacturonases PG represent an important member of pectinases group of enzymes with immense industrial applications. A fungal strain Aspergillus niger MTCC478 was used for the production of polygalacturonase both under submerged and solid-state fermentation condition. Further its production was optimized under solid-state fermentation condition with media comprising of wheat bran and tea extract. Purification of an exo-PG was achieved by acetone precipitation 60–90% and CM-cellulose column chromatography revealing 15.28-fold purification with a specific activity of 33.47 U-mg protein and 1.2% yield. A relative molecular mass of purified PG was approximately 124.0 kDa. The pH and temperature optimum was found to be 4 and 50 °C, respectively. The kcat and Km value for degradation of PGA by the purified enzyme was found to be 194 s and 2.3 mg-mL, respectively. Cu was found to enhance the PG activity while Ag completely inhibited the enzyme activity. The application of the purified PG in orange juice clarification was elucidated.

KeywordsPolygalacturonase Aspergillus niger MTCC478 Exo-PG Fruit juice clarification Solid-state fermentation 



Autor: Gautam Anand - Sangeeta Yadav - Dinesh Yadav

Fuente: https://link.springer.com/







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