Expression Profiling of Selected Glutathione Transferase Genes in Zea mays L. Seedlings Infested with Cereal AphidsReportar como inadecuado




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The purpose of this report was to evaluate the expression patterns of selected glutathione transferase genes gst1, gst18, gst23 and gst24 in the tissues of two maize Zea mays L. varieties relatively resistant Ambrozja and susceptible Tasty Sweet that were colonized with oligophagous bird cherry-oat aphid Rhopalosiphum padi L. or monophagous grain aphid Sitobion avenae L

Simultaneously, insect-triggered generation of superoxide anion radicals O2•− in infested Z. mays plants was monitored. Quantified parameters were measured at 1, 2, 4, 8, 24, 48 and 72 h post-initial aphid infestation hpi in relation to the non-infested control seedlings. Significant increases in gst transcript amounts were recorded in aphid-stressed plants in comparison to the control seedlings. Maximal enhancement in the expression of the gst genes in aphid-attacked maize plants was found at 8 hpi gst23 or 24 hpi gst1, gst18 and gst24 compared to the control. Investigated Z. mays cultivars formed excessive superoxide anion radicals in response to insect treatments, and the highest overproduction of O2•− was noted 4 or 8 h after infestation, depending on the aphid treatment and maize genotype. Importantly, the Ambrozja variety could be characterized as having more profound increments in the levels of gst transcript abundance and O2•− generation in comparison with the Tasty Sweet genotype.



Autor: Hubert Sytykiewicz , Grzegorz Chrzanowski, Paweł Czerniewicz, Iwona Sprawka, Iwona Łukasik, Sylwia Goławska, Cezary Sempruch

Fuente: http://plos.srce.hr/



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