Enhanced Tissue Factor Expression by Blood Eosinophils from Patients with Hypereosinophilia: A Possible Link with ThrombosisReportar como inadecuado




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Thrombotic risk is increased in eosinophil-mediated disorders, and several hypotheses have been proposed to link eosinophilia and thrombosis. In particular, eosinophils have been described as source of tissue factor TF, the main initiator of blood coagulation; however, this aspect is still controversial. This study was aimed to evaluate whether TF expression varies in eosinophils isolated from normal subjects and patients with different hypereosinophilic conditions. Eosinophils were immunologically purified from peripheral blood samples of 9 patients with different hypereosinophilic conditions and 9 normal subjects. Western blot analysis and real-time polymerase chain reaction RT-PCR were performed to test eosinophil TF expression. For comparison, TF expression was evaluated in monocytes from blood donors and in human endothelial ECV304 and fibroblast IMR90 cell lines. Western blot analysis revealed a major band of 47,000 corresponding to native TF in homogenates of purified eosinophils with a higher intensity in the 9 patients than in the 9 controls p<0.0001. According to RT-PCR cycle threshold Ct, TF gene expression was higher in eosinophils from patients than in those from controls, median range 35.10 19.45–36.50 vs 37.17 35.33–37.87 p = 0.002, and was particularly abundant in one patient with idiopathic hypereosinophilic syndrome and ischemic heart attacks Ct: 19.45. TF gene expression was moderate in monocytes, Ct: 31.32 29.82–33.49 and abundant in endothelial cells, Ct: 28.70 27.79–29.57 and fibroblasts, Ct: 22.77 19.22–25.05. Our results indicate that human blood eosinophils contain variable amounts of TF. The higher TF expression in patients with hypereosinophilic disorders may contribute to increase the thrombotic risk.



Autor: Massimo Cugno , Angelo V. Marzano, Maurizio Lorini, Vincenzo Carbonelli, Alberto Tedeschi

Fuente: http://plos.srce.hr/



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