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Plant Reproduction

, Volume 30, Issue 2, pp 81–94

First Online: 16 May 2017Received: 21 November 2016Accepted: 02 May 2017DOI: 10.1007-s00497-017-0301-6

Cite this article as: Paupière, M.J., Müller, F., Li, H. et al. Plant Reprod 2017 30: 81. doi:10.1007-s00497-017-0301-6 Key messagePollen development metabolomics.

AbstractDeveloping pollen is among the plant structures most sensitive to high temperatures, and a decrease in pollen viability is often associated with an alteration of metabolite content. Most of the metabolic studies of pollen have focused on a specific group of compounds, which limits the identification of physiologically important metabolites. To get a better insight into pollen development and the pollen heat stress response, we used a liquid chromatography–mass spectrometry platform to detect secondary metabolites in pollen of tomato Solanum lycopersicum L. at three developmental stages under control conditions and after a short heat stress at 38 °C. Under control conditions, the young microspores accumulated a large amount of alkaloids and polyamines, whereas the mature pollen strongly accumulated flavonoids. The heat stress treatment led to accumulation of flavonoids in the microspore. The biological role of the detected metabolites is discussed. This study provides the first untargeted metabolomic analysis of developing pollen under a changing environment that can serve as reference for further studies.

KeywordsPollen development Metabolomics Untargeted analysis Heat stress High temperature Communicated by Dolf Weijers.

Electronic supplementary materialThe online version of this article doi:10.1007-s00497-017-0301-6 contains supplementary material, which is available to authorized users.

Autor: Marine J. Paupière - Florian Müller - Hanjing Li - Ivo Rieu - Yury M. Tikunov - Richard G. F. Visser - Arnaud G. Bov

Fuente: https://link.springer.com/

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