Endothelium Trans Differentiated from Whartons Jelly Mesenchymal Cells Promote Tissue Regeneration: Potential Role of Soluble Pro-Angiogenic FactorsReportar como inadecuado




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Background

Mesenchymal stem cells have a high capacity for trans-differentiation toward many adult cell types, including endothelial cells. Feto-placental tissue, such as Wharton-s jelly is a potential source of mesenchymal stem cells with low immunogenic capacity; make them an excellent source of progenitor cells with a potential use for tissue repair. We evaluated whether administration of endothelial cells derived from mesenchymal stem cells isolated from Wharton-s jelly hWMSCs can accelerate tissue repair in vivo.

Methods

Mesenchymal stem cells were isolated from human Wharton-s jelly by digestion with collagenase type I. Endothelial trans-differentiation was induced for 14 hWMSC-End14d and 30 hWMSC-End30d days. Cell phenotyping was performed using mesenchymal CD90, CD73, CD105 and endothelial Tie-2, KDR, eNOS, ICAM-1 markers. Endothelial trans-differentiation was demonstrated by the expression of endothelial markers and their ability to synthesize nitric oxide NO.

Results

hWMSCs can be differentiated into adipocytes, osteocytes, chondrocytes and endothelial cells. Moreover, these cells show high expression of CD73, CD90 and CD105 but low expression of endothelial markers prior to differentiation. hWMSCs-End express high levels of endothelial markers at 14 and 30 days of culture, and also they can synthesize NO. Injection of hWMSC-End30d in a mouse model of skin injury significantly accelerated wound healing compared with animals injected with undifferentiated hWMSC or injected with vehicle alone. These effects were also observed in animals that received conditioned media from hWMSC-End30d cultures.

Conclusion

These results demonstrate that mesenchymal stem cells isolated from Wharton-s jelly can be cultured in vitro and trans-differentiated into endothelial cells. Differentiated hWMSC-End may promote neovascularization and tissue repair in vivo through the secretion of soluble pro-angiogenic factors.



Autor: Valeria Aguilera, Luis Briceño, Hector Contreras, Liliana Lamperti, Esperanza Sepúlveda, Francisca Díaz-Perez, Marcelo León,

Fuente: http://plos.srce.hr/



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