Increases in the Numerical Density of GAT-1 Positive Puncta in the Barrel Cortex of Adult Mice after Fear ConditioningReportar como inadecuado




Increases in the Numerical Density of GAT-1 Positive Puncta in the Barrel Cortex of Adult Mice after Fear Conditioning - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Three days of fear conditioning that combines tactile stimulation of a row of facial vibrissae conditioned stimulus, CS with a tail shock unconditioned stimulus, UCS expands the representation of -trained- vibrissae, which can be demonstrated by labeling with 2-deoxyglucose in layer IV of the barrel cortex. We have also shown that functional reorganization of the primary somatosensory cortex S1 increases GABAergic markers in the hollows of -trained- barrels of the adult mouse. This study investigated how whisker-shock conditioning CS+UCS affected the expression of puncta of a high-affinity GABA plasma membrane transporter GAT-1 in the barrel cortex of mice 24 h after associative learning paradigm. We found that whisker-shock conditioning CS+UCS led to increase expression of neuronal and astroglial GAT-1 puncta in the -trained- row compared to controls: Pseudoconditioned, CS-only, UCS-only and Naïve animals. These findings suggest that fear conditioning specifically induces activation of systems regulating cellular levels of the inhibitory neurotransmitter GABA.



Autor: Ewa Siucinska , Adam Hamed, Malgorzata Jasinska

Fuente: http://plos.srce.hr/



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