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Spectroscopy: An International JournalVolume 27 2012, Issue 5-6, Pages 399-402

Centro de Lasers e Aplicações, Instituto de Pesquisas Energéticas e Nucleares-CNEN-SP, Avenida Professor Lineu Prestes 2242, Cid. Universitária, 05508-000 São Paulo, SP, Brazil

Departamento de Patologia, Faculdade de Medicina Veterinaria e Zootecnia, Universidade de São Paulo, Avenida Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-900 São Paulo, SP, Brazil

Copyright © 2012 Thiago Martini Pereira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the present study is to evaluate the differences on FTIR spectra of the normal lung cell noncancerous mice lung epithelial cell line e10 due to different fixation protocols for histological processing. The results shown that formalin and methacarn normally used in fixation did cause many changes on the FTIR spectra of mice lung cells e10, mainly in the organic compounds 800–1800 cm

in lipids, DNA, and proteins, and the alcohol 70% fixation protocol caused almost no changes on the FTIR spectra compared to unfixed cells spectra in PBS. It can be concluded that histological processing with alcohol 70% fixation protocol can be used in the FTIR study of mice lung cell line e10.





Autor: Thiago Martini Pereira, Maria Lucia Zaidan Dagli, Grégory Mennecier, and Denise Maria Zezell

Fuente: https://www.hindawi.com/



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