The epigenetic memory of temperature during embryogenesis modifies the expression of bud burst-related genes in Norway spruce epitypesReportar como inadecuado




The epigenetic memory of temperature during embryogenesis modifies the expression of bud burst-related genes in Norway spruce epitypes - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Planta

pp 1–14

First Online: 02 June 2017Received: 13 February 2017Accepted: 23 May 2017DOI: 10.1007-s00425-017-2713-9

Cite this article as: Carneros, E., Yakovlev, I., Viejo, M. et al. Planta 2017. doi:10.1007-s00425-017-2713-9

Abstract

Main conclusionEpigenetic memory affects the timing of bud burst phenology and the expression of bud burst-related genes in genetically identical Norway spruce epitypes in a manner usually associated with ecotypes.

In Norway spruce, a temperature-dependent epigenetic memory established during embryogenesis affects the timing of bud burst and bud set in a reproducible and predictable manner. We hypothesize that the clinal variation in these phenological traits, which is associated with adaptation to growth under frost-free conditions, has an epigenetic component. In Norway spruce, dehydrins DHNs have been associated with extreme frost tolerance. DHN transcript levels decrease gradually prior to flushing, a time when trees are highly sensitive to frost. Furthermore, EARLY BUD BREAK 1 genes EBB1 and the FT-TFL1-LIKE 2-gene PaFTL2 were previously suggested to be implied in control of bud phenology. Here we report an analysis of transcript levels of 12 DHNs, 3 EBB1 genes and FTL2 in epitypes of the same genotype generated at different epitype-inducing temperatures, before and during spring bud burst. Earlier flushing of epitypes originating from embryos developed at 18 °C as compared to 28 °C, was associated with differential expression of these genes between epitypes and between buds and last year’s needles. The majority of these genes showed significantly different expressions between epitypes in at least one time point. The general trend in DHN expression pattern in buds showed the expected reduction in transcript levels when approaching flushing, whereas, surprisingly, transcript levels peaked later in needles, mainly at the moment of bud burst. Collectively, our results demonstrate that the epigenetic memory of temperature during embryogenesis affects bud burst phenology and expression of the bud burst-related DHN, EBB1 and FTL2 genes in genetically identical Norway spruce epitypes.

KeywordsBud phenology Dehydrins EBB1 genes Epigenetic memory FTL2 Picea abies AbbreviationsCECold embryogenesis environment 18 °C

DHNsDehydrins

EBB1EARLY BUD BREAK 1-genes

FTL2FT-TFL1-LIKE 2-gene

PCAPrincipal component analysis

WEWarm embryogenesis environment 28 °C

Electronic supplementary materialThe online version of this article doi:10.1007-s00425-017-2713-9 contains supplementary material, which is available to authorized users.





Autor: Elena Carneros - Igor Yakovlev - Marcos Viejo - Jorunn E. Olsen - Carl Gunnar Fossdal

Fuente: https://link.springer.com/







Documentos relacionados