iTRAQ-based proteome profile analysis of superior and inferior Spikelets at early grain filling stage in japonica RiceReportar como inadecuado

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BMC Plant Biology

, 17:100

Development and cell biology


BackgroundLarge-panicle rice varieties often fail to achieve their yield potential due to poor grain filling of late-flowering inferior spikelets IS. The physiological and molecular mechanisms of poor IS grain filling, and whether an increase in assimilate supply could regulate protein abundance and consequently improve IS grain filling for japonica rice with large panicles is still partially understood.

ResultsA field experiment was performed with two spikelet removal treatments at anthesis in the large-panicle japonica rice line W1844, including removal of the top 1-3 of spikelets T1 and removal of the top 2-3 of spikelets T2, with no spikelet removal as a control T0. The size, weight, setting rate, and grain filling rate of IS were significantly increased after spikelet removing. The biological functions of the differentially expressed proteins DEPs between superior and inferior spikelets as well as the response of IS to the removal of superior spikelets SS were investigated by using iTRAQ at 10 days post anthesis. A total of 159, 87, and 28 DEPs were identified from group A T0-SS-T0-IS, group B T0-SS-T2-IS, and group C T2-IS-T0-IS, respectively. Among these, 104, 63, and 22 proteins were up-regulated, and 55, 24, and 6 proteins were down-regulated, respectively. Approximately half of these DEPs were involved in carbohydrate metabolism sucrose-to-starch metabolism and energy metabolism and protein metabolism protein synthesis, folding, degradation, and storage.

ConclusionsReduced endosperm cell division and decreased activities of key enzymes associated with sucrose-starch metabolism and nitrogen metabolism are mainly attributed to the poor sink strength of IS. In addition, due to weakened photosynthesis and respiration, IS are unable to obtain a timely supply of materials and energy after fertilization, which might be resulted in the stagnation of IS development. Finally, an increased abundance of 14–3-3 protein in IS could be involved in the inhibition of starch synthesis. The removal of SS contributed to transfer of assimilates to IS and enhanced enzymatic activities of carbon metabolism sucrose synthase, starch branching enzyme, soluble starch synthase, and pullulanase and nitrogen metabolism aspartate amino transferase and alanine amino transferase, promoting starch and protein synthesis in IS. In addition, improvements in energy metabolism greater abundance of pyrophosphate-fructose 6-phosphate 1-phosphotransferase might be played a vital role in inducing the initiation of grain filling. These results collectively demonstrate that carbohydrate supply is the main cause of poor IS grain filling.

KeywordsRice Removal of superior spikelets Inferior spikelets Grain filling iTRAQ Proteome Abbreviations2-DEtwo-dimensional gel electrophoresis

ABAAbscisic acid

ADPGAdenosine diphosphate glucose

AGPaseADP-glucose pyrophosphorylase

DEPsDifferentially expressed proteins

DPADays post anthesis

GOTAspartate amino transferase

GPTAlanine amino transferase

IAAIndole-3-acetic acid

ISInferior spikelets

ITRAQIsobaric tags for relative and absolute quantitation

MSMedium spikelets

PFPPyrophosphate-fructose 6-phosphate 1-phosphotransferase


SBEStarch branching enzyme

SSSuperior spikelets

SSSSoluble starch synthase

SuSaseSucrose synthase

TCATricarboxylic acid

UDPGUridine diphosphoglucose

Electronic supplementary materialThe online version of this article doi:10.1186-s12870-017-1050-2 contains supplementary material, which is available to authorized users.

Autor: Cuicui You - Lin Chen - Haibing He - Liquan Wu - Shaohua Wang - Yanfeng Ding - Chuanxi Ma


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