Monocrotophos Pesticide Decreases the Plasma Levels of Total 3,3′,5-Triiodo-l-Thyronine and Alters the Expression of Genes Associated with the Thyroidal Axis in Female Goldfish Carassius auratusReportar como inadecuado




Monocrotophos Pesticide Decreases the Plasma Levels of Total 3,3′,5-Triiodo-l-Thyronine and Alters the Expression of Genes Associated with the Thyroidal Axis in Female Goldfish Carassius auratus - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Our recent study showed that monocrotophos MCP pesticide disrupted the hypothalamic-pituitary-thyroid HPT axis in male goldfish Carassius auratus; however, the effects of MCP on the thyroid system in female goldfish are remain unclear. In the present study, plasma thyroid hormone TH and thyroid-stimulating hormone TSH levels were evaluated in female goldfish exposed to 0.01, 0.10, and 1.00 mg-L of 40% MCP-based pesticide for 21 days in a semi-static exposure system. Expression profiles of HPT axis-responsive genes, including transthyretin ttr, deiodinases d1, d2, and d3, tshβ, thyrotropin-releasing hormone trh, and corticotrophin-releasing hormone crh, were determined. The results indicated that MCP decreased the plasma levels of total 3,3′,5-triiodo-l-thyronine TT3 and the ratio of TT3 to total 3,3′,5,5′-l-thyroxine TT4, and induced alternative expression of TH-related genes. Exposure to 0.01 and 0.10 mg-L MCP pesticide resulted in the up-regulation of ttr mRNA. The reduction of plasma TT3 levels was partly attributed to an increase in the metabolism of T3 in the liver, as revealed by the highly elevated hepatic d1 and d3 mRNA levels in the MCP treatment groups, and the expression of hepatic d3 showed a negative correlation with the plasma TT3-TT4 levels in females. Moreover, the plasma TSH levels were lower in females exposed to 0.01 and 0.10 mg-L MCP pesticide, whereas the up-regulation of tshβ mRNA levels was compensated by the decreased plasma TT3 levels. These results indicated that MCP had the potential to influence several pathways of HPT axis homeostasis in female goldfish.



Autor: Xiaona Zhang, Hua Tian, Wei Wang, Shaoguo Ru

Fuente: http://plos.srce.hr/



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