Calreticulin localizes to plant intra-extracellular peripheries of highly specialized cells involved in pollen-pistil interactionsReportar como inadecuado




Calreticulin localizes to plant intra-extracellular peripheries of highly specialized cells involved in pollen-pistil interactions - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Protoplasma

pp 1–11

First Online: 15 June 2017Received: 21 March 2017Accepted: 05 June 2017DOI: 10.1007-s00709-017-1134-8

Cite this article as: Wasąg, P., Suwińska, A., Zakrzewski, P. et al. Protoplasma 2017. doi:10.1007-s00709-017-1134-8

Abstract

Calcium Ca plays essential roles in generative reproduction of angiosperms, but the sites and mechanisms of Ca storage and mobilization during pollen-pistil interactions have not been fully defined. Both external and internal Ca stores are likely important during male gametophyte communication with the sporophytic and gametophytic cells within the pistil. Given that calreticulin CRT, a Ca-buffering protein, is able to bind Ca reversibly, it can serve as a mobile store of easily releasable Ca so called an exchangeable Ca in eukaryotic cells. CRT has typical endoplasmic reticulum ER targeting and retention signals and resides primarily in the ER. However, localization of this protein outside the ER has also been revealed in both animal and plant cells, including Golgi-dictyosomes, nucleus, plasma membrane-cell surface, plasmodesmata, and even extracellular matrix. These findings indicate that CRT may function in a variety of different cell compartments and specialized structures. We have recently shown that CRT is highly expressed and accumulated in the ER of plant cells involved in pollen-pistil interactions in Petunia, and we proposed an essential role for CRT in intracellular Ca storage and mobilization during the key reproductive events. Here, we demonstrate that both CRT and exchangeable Ca are localized in the intra-extracellular peripheries of highly specialized plant cells, such as the pistil transmitting tract cells, pollen tubes, nucellus cells surrounding the embryo sac, and synergids. Based on our present results, we propose that extracellularly located CRT is also involved in Ca storage and mobilization during sexual reproduction of angiosperms.

KeywordsCell wall Exchangeable Ca Filiform apparatus Plasmodesmata Pollen tube Style transmitting tissue AbbreviationsCaCalcium-calcium ions

Ca pptsCa-antimonate precipitates

Cal MAbMonoclonal antibody against callose

CRTCalreticulin

CRT PAbPolyclonal antibody against plant CRT

EREndoplasmic reticulum

P. Wasąg and A. Suwińska contributed equally to this work

Handling Editor: Liwen Jiang





Autor: Piotr Wasąg - Anna Suwińska - Przemysław Zakrzewski - Jakub Walczewski - Robert Lenartowski - Marta Lenartowska

Fuente: https://link.springer.com/







Documentos relacionados