Hemolytic Streptococcus May Exacerbate Kidney Damage in IgA Nephropathy through CCL20 Response to the Effect of Th17 CellsReportar como inadecuado




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Background

The exacerbation of IgA nephropathy IgAN is related to respiratory tract infection with hemolytic streptococcus HS, but the mechanism is unknown. In this study we investigated the role of chemokine ligand 20 CCL20 in response to the effect of T helper 17 Th17 cells in the pathogenesis of IgAN associated with HS.

Methods

Thirty mice were randomly divided into five groups: control mice control, IgAN mice IgAN, HS-infected IgAN mice HS-IgAN, CCL20-treated IgAN mice CCL20-IgAN, and CCL20-treated HS infected IgAN mice CCL20-HS-IgAN. IgAN mice were induced with lipopolysaccharide, carbon tetrachloride and bovine serum albumin. Then the mice were sensitized with CCL20 antibody and infected with alpha-hemolytic streptococcus α-HS isolated from tonsils in sequence. Urine Albumin-Creatinine ratio and sediments were measured. The pathological changes in kidney and lung tissues were observed under microscopy. Th17 cells and regulatory T cells Tregs in kidneys were tested by flow cytometry. CCL20, IL-17A, IL-6 and IL-21 in the kidneys were detected by ELISA.

Results

The IgAN mice had albuminuria and microscopic hematuria, renal mesangial proliferation, IgA deposition, high electron dense deposition in glomerular mesangial region, decreased frequency of Tregs, increased frequency of Th17 and Th17-Treg ratio. Furthermore, Th17-related cytokines CCL20, IL-17A, IL-6 and IL-21 were all increased in the kidneys of IgAN mice. Compared with IgAN mice, the manifestations in HS-IgAN mice were more severe, but alleviated in CCL20-treated groups.

Conclusion

α-HS may exacerbate kidney damage in IgAN through CCL20 response to the effect of Th17 cells.



Autor: Ting Meng , Xiaozhao Li , Xiang Ao, Yong Zhong, Rong Tang, Weisheng Peng, Jinghua Yang, Mingxiang Zou, Qiaoling Zhou

Fuente: http://plos.srce.hr/



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