Assessment of the circulating cell-free DNA marker association with diagnosis and prognostic prediction in patients with lymphoma: a single-center experienceReportar como inadecuado




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Annals of Hematology

pp 1–9

First Online: 16 June 2017Received: 27 November 2016Accepted: 06 June 2017DOI: 10.1007-s00277-017-3043-5

Cite this article as: Li, M., Jia, Y., Xu, J. et al. Ann Hematol 2017. doi:10.1007-s00277-017-3043-5

Abstract

Circulating cell-free DNA ccfDNA has been shown to be associated with the clinical characteristics and prognosis of cancer patients. Our objective was to assess whether the concentration and integrity index of ccfDNA in plasma may be useful for diagnosing and monitoring the progression of patients with lymphoma. We included plasma samples from 174 lymphoma patients and 80 healthy individuals. The total concentration of ccfDNA was determined using a fluorometry method, and the DNA integrity index DII, which is the ratio of longer to shorter DNA fragments, for the APP gene was detected using real-time quantitative PCR. The median levels of the ccfDNA concentration and the DII in patients with lymphoma were significantly higher than those in controls both P < 0.0001. Increases in the ccfDNA concentration and the DII were associated with advanced stage disease, elevated lactate dehydrogenase levels, and a higher prognosis score. In patients with diffuse large B cell lymphoma DLBCL, high levels of ccfDNA both concentration and the DII showed an inferior 2-year progression-free survival PFS P = 0.001; P < 0.0001, respectively. Our study provides quantitative and qualitative evidence in favor of using ccfDNA analysis in lymphoma patients for diagnostic and prognostic assessments.

KeywordsLymphoma Circulating DNA Integrity Prognosis 



Autor: Mao Li - Yongqian Jia - Juan Xu - Xiaomin Cheng - Caigang Xu

Fuente: https://link.springer.com/







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